FUNGuild is a Python-based, open-access tool that automatically assigns ecological guilds to fungal taxa. Its purpose is to help researchers better understand the functional roles of fungi by providing a standardized method for analyzing fungal community datasets, regardless of the sequencing platform or analysis pipeline used. This tool is designed for anyone, from ecologists or mycologists to bioinformaticians and conservation biologists, who work with fungal environmental sequencing data and need to interpret the ecological significance.